Ames offers a pedestrian-friendly environment, particularly in areas near Iowa State University and the downtown district. The city features a network of sidewalks and shared-use paths that make walking a convenient option for accessing many destinations. Compact neighborhoods and well-maintained infrastructure support ease of movement on foot. However, weather conditions, especially during colder months, may impact comfort and safety for pedestrians. While walking is practical for shorter distances, some areas of the city may require alternative transportation due to greater spacing between destinations.

Cycling in Ames is supported by a range of biking infrastructure, making it a practical way to get around, particularly in areas like the Iowa State University campus, which features bike lanes and paths. The city includes bike-friendly routes and facilities such as bike lockers at the Ames Intermodal Facility. While biking is convenient for shorter distances, the ease of access to destinations may vary depending on the area. Weather conditions and seasonal changes could also impact biking comfort and safety, so planning ahead is important.

The public transit system in Ames is primarily served by a bus network that connects key areas, including Iowa State University and other parts of the city. The routes are designed to make it convenient to reach many destinations, especially within the central parts of Ames. The infrastructure supports regular and accessible bus services, which can be a practical option for getting around. However, factors such as distance to stops and seasonal weather conditions may influence the ease of using public transit.